Overview

  • Product name

    Mouse Chemerin ELISA Kit
    See all Chemerin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 8 1.78%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 2.49%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.75 pg/ml
  • Range

    14.06 pg/ml - 900 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 86% - 101%
    Cell culture media 100 96% - 102%
    Heparin Plasma 102 100% - 106%
    EDTA Plasma 112 105% - 118%
    Citrate Plasma 92 92% - 93%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Chemerin mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Chemerin protein in serum, plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Mouse Chemerin (also known as retinoic acid receptor responder protein 2, or Rarres2) is an adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Chemerin positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Furthermore, Chemerin also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab204520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse Chemerin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Native mouse Chemerin was measured in 0.5% mouse serum and 0.66% PHA + PMA stimulated L929 cultured media that were diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Chemerin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Native mouse Chemerin was measured in 0.33% mouse plasma citrate, 0.5% mouse plasma EDTA and 0.66% mouse plasma heparin that were diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • During the exponential growth phase, L929 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. Samples were diluted 1:300 in Sample Diluent NS. The concentrations of mouse Chemerin were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). Note that media only gave an O.D. equivalent to background.

Protocols

References

ab204520 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Mouse Chemerin ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Very impressed with this ELISA, always quick and easy to run. Ran at 1000 fold dilution in C57BL6 mouse serum and our values were right in the middle of the curve. 1000 fold dilution for mouse serum seems to be optimal after running this several times.

Mr. Joshua Preston

Verified customer

Submitted Jun 25 2019

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