Product nameMouse Chemokine Antibody Array (Membrane, 25 Targets)
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Mouse
Abcam's Mouse Chemokine Antibody Array (ab211067) for use with serum, plasma, cell culture media, cell lysate, tissue lysate and other liquid sample types.
Targets: 6Ckine (CCL21), BLC (CXCL13), CTACK (CCL27), CXCL16, Eotaxin-1 (CCL11), Eotaxin-2 (CCL24), Fractalkine (CX3CL1), I-TAC (CXCL11), KC (CXCL1), LIX, MCP-1 (CCL2), MCP-5, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), PF-4 (CXCL4), RANTES (CCL5), SDF-1 (CXCL12), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25).
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 2 vials 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab211067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab211067 has not yet been referenced specifically in any publications.