Mouse CHIT1 ELISA Kit (ab246542)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse CHIT1 ELISA Kit
See all CHIT1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.5% Inter-assay Sample n Mean SD CV% Serum 3 2.9% -
Sample type
Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.911 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 98% - 106% Tissue Culture Media 105 96% - 115% Hep Plasma 101 96% - 104% EDTA Plasma 99 95% - 103% Cit plasma 91 85% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
CHIT1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CHIT1 protein in mouse serum, plasma, and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
CHIT1 (Chitotriosidase) a member of the chitinase family, is secreted as 50 kDa protein encoded by the CHIT1 gene. CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form. CHIT1 degrades chitin, chitotriose and chitobiose. It is selectively expressed in activated tissue macrophages and may participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Very high plasma levels of CHIT1 are found in patients with Gaucher disease type 1. CHIT1 is a known biomarker in the monitoring of Gaucher disease. CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke. Mouse CHIT1 shares 74.8%, 81.9%, and 49.2% sequence homology with human, rat, and bovine respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CHIT1 Capture Antibody 1 x 600µl 10X Mouse CHIT1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CHIT1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. -
Tissue specificity
Detected in spleen. Secreted by cultured macrophages. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Contains 1 chitin-binding type-2 domain. -
Cellular localization
Secreted. Lysosome. A small proportion is lysosomal. - Information by UniProt
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Alternative names
- CHI3
- CHIT
- CHIT1
see all -
Database links
- Entrez Gene: 71884 Mouse
- SwissProt: Q9D7Q1 Mouse
- Unigene: 328268 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse CHIT1 ELISA Kit (ab246542)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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. Example of mouse CHIT1 standard curve in Sample Diluent NS.The CHIT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Interpolated concentrations of native CHIT1 in mouse serum and plasma.The concentrations of CHIT1 were measured in duplicates, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.5%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, and plasma (heparin) 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 131.15 ng/mL in serum, 128.06 ng/mL in plasma (citrate), 105.87 ng/mL in plasma (EDTA), and 162.76 ng/mL in plasma (heparin).
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Interpolated concentrations of native CHIT1 in mouse tissue culture supernatant samples.The concentrations of CHIT1 were measured in duplicate, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted spleen supernatant is 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 422.68 pg/mL in spleen supernatant.
Protocols
References
ab246542 has not yet been referenced specifically in any publications.
