Key features and details
- Sensitivity: 49 pg/ml
- Range: 49.15 pg/ml - 12000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Chordin ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeSerum, Cell culture media, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range49.15 pg/ml - 12000 pg/ml
Sample specific recovery Sample type Average % Range Serum 74 68% - 86% Plasma 74 68% - 85% Cell culture media 100 80% - 117%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse Chordin ELISA Kit is designed for the quantitative determination of Chordin in cell culture supernatants, plasma and serum samples.
ΔNote: Use Citrate and EDTA as anti-coagulant. Heparin is not recommended.
This assay employs an antibody specific for mouse Chordin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chordin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Chordin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chordin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 4-1BB, 6Ckine, ACE, ALK-1, AREG, CT-1 , CD27, CD27 Ligand, CD36, CD40 Ligand, CTLA 4, Decorin, DKK-1, E Cadherin, EGF, Endoglin, Epigen, EPR, Galectin 1, GAS 1, GAS 6, GITR Ligand, Granzyme B, HAI-1, HGF, IL-1R4, IL-11, IL-17B, IL-17E, IL-17 F, IL-1RA, IL-2R alpha, IL-20, IL-21, IL-28, IL-6R, JAM-A, MFG-E8, NEP, PTX3, PRL, RAGE, TACI, TREM, TWEAK.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse Chordin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse Chordin Detection Antibody 2 vials Mouse Chordin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionDorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Tissue specificityExpressed at the highest level in liver.
Sequence similaritiesBelongs to the chordin family.
Contains 4 CHRD domains.
Contains 4 VWFC domains.
modificationsCleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development.
- Information by UniProt
ab267579 has not yet been referenced specifically in any publications.