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Immunology Innate Immunity Complement Classical Pathway
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Mouse Complement C3 ELISA Kit (ab157711)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (17)

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Other sample results
  • Sample results
  • Standard Curve

Key features and details

  • Sensitivity: 1.6578 ng/well
  • Range: 3.13 ng/ml - 200 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Complement C3 ELISA Kit
    See all C3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.6578 ng/well
  • Range

    3.13 ng/ml - 200 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Product overview

    Mouse Complement C3 ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Complement C3 in mouse biological samples.


    In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-mouse Complement C3 antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Mouse Complement C3 Calibrator (lyophilized) 1 vial
    Mouse Complement C3 ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01024 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Acylation stimulating protein cleavage product
    • AHUS5
    • ARMD9
    • ASP
    • C3
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • c3 complement
    • C3adesArg
    • CO3_HUMAN
    • Complement C3
    • Complement C3 alpha chain
    • Complement C3b alpha' chain
    • Complement C3c alpha' chain fragment 1
    • Complement C3c alpha' chain fragment 2
    • Complement C3c alpha'' chain fragment 2
    • Complement C3d fragment
    • Complement C3dg fragment
    • Complement C3f fragment
    • Complement C3g fragment
    • Complement component 3
    • Complement factor 3
    • CPAMD1
    • HEL S 62p
    • omplement C3 beta chain
    see all
  • Database links

    • Entrez Gene: 12266 Mouse
    • Entrez Gene: 24232 Rat
    • SwissProt: P01027 Mouse
    • SwissProt: P01026 Rat
    • Unigene: 19131 Mouse
    • Unigene: 11378 Rat

    Associated products

      Images

      • Other sample results
        Other sample results

        Complement C3 measured in tissue extracts (total protein concentration: Ms liver - 17.46 mg/mL, Ms kidney - 15.6 mg/mL; prepared in RIPA buffer with protease inhibitor) and cell culture supernatants showing quantity (ng) per mL of tested sample. Tissue samples were diluted 100-10000 fold. Supernatants were diluted 1-10 fold.

      • Sample results
        Sample results

        Complement C3 measured in biological fluids of healthy species showing quantity (ng) per mL of tested sample. Samples were diluted 8000-128000 fold.

      • Standard Curve
        Standard Curve

        Representative standard curve using ab157711 Complement C3 Mouse ELISA kit.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (17)

      Publishing research using ab157711? Please let us know so that we can cite the reference in this datasheet.

      ab157711 has been referenced in 17 publications.

      • Park JW  et al. Compensatory role of C3 convertase on the strain difference for C3 protein expression in FVB/N, C3H/HeN and C57BL/6N mice. Lab Anim Res 36:4 (2020). PubMed: 32206611
      • Zha H  et al. Intracellular Activation of Complement C3 Leads to PD-L1 Antibody Treatment Resistance by Modulating Tumor-Associated Macrophages. Cancer Immunol Res 7:193-207 (2019). PubMed: 30514794
      • Rathore AP  et al. Dengue virus-elicited tryptase induces endothelial permeability and shock. J Clin Invest 129:4180-4193 (2019). PubMed: 31265436
      • Royer DJ  et al. Complement and CD4+ T cells drive context-specific corneal sensory neuropathy. Elife 8:N/A (2019). PubMed: 31414985
      • Zhang Z  et al. Upconversion superballs for programmable photoactivation of therapeutics. Nat Commun 10:4586 (2019). PubMed: 31594932
      View all Publications for this product

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