Overview

  • Product name

    Mouse Complement C5a ELISA Kit
    See all Complement C5 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.5 pg/ml
  • Range

    1.5 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 133.4 127% - 140%
    Plasma 128.5 112% - 140%
    Cell culture media 132 121% - 147%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Complement C5a mouse ELISA Kit (ab193718) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Complement C5a in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Complement C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Complement C5a present in the samples. The wells are washed and biotinylated anti-mouse Complement C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Complement C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated mouse C5a detection antibody (lyophilized) 2 vials
    Mouse C5a Standard (Lyophilized) 2 vials
    Pre-coated mouse C5a Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in disease

    Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • CO5_HUMAN
    • complement C5
    • Complement C5 alpha'' chain
    • Complement component 5
    • CPAMD4
    • ECLZB
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve in Assay Diluent A using ab193718 Complement C5a Mouse ELISA Kit.

  • Typical Standard Curve in Assay Diluent B using ab193718 Complement C5a Mouse ELISA Kit.

Protocols

References

This product has been referenced in:

  • Wang X  et al. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. J Control Release 293:201-214 (2019). Read more (PubMed: 30527753) »
  • Serré J  et al. Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency. Data Brief 22:703-708 (2019). Read more (PubMed: 30656202) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

For the ab193718 Mouse Complement C5a ELISA Kit, the antibodies used in the kit detect the C5a portion, but that doesn’t rule out the possibility of it also recognizing non-cleaved C5 since the region is included in the non-cleaved portion too. There could be some steric hindrance, but we have not determined that ourselves, and would need to be tested.


Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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