Product nameMouse Complement C5a ELISA Kit
See all Complement C5 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range1.5 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Serum 133.4 127% - 140% Plasma 128.5 112% - 140% Cell culture media 132 121% - 147%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Complement C5a mouse ELISA Kit (ab193718) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Complement C5a in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Complement C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Complement C5a present in the samples. The wells are washed and biotinylated anti-mouse Complement C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Complement C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated mouse C5a detection antibody (lyophilized) 2 vials Mouse C5a Standard (Lyophilized) 2 vials Pre-coated mouse C5a Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
Involvement in diseaseDefects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
- Information by UniProt
- anaphylatoxin C5a analog
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
- SwissProt: P06684 Mouse
Our Abpromise guarantee covers the use of ab193718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Wang X et al. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. J Control Release 293:201-214 (2019). Read more (PubMed: 30527753) »
- Serré J et al. Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency. Data Brief 22:703-708 (2019). Read more (PubMed: 30656202) »