Mouse CSF-1-R ELISA Kit (ab240681)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse CSF-1-R ELISA Kit
See all CSF-1-R kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.8% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.04 ng/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 85% - 97% Serum 108 102% - 111% Cell culture extracts 118 116% - 119% Tissue Extracts 118 111% - 132% Hep Plasma 105 101% - 108% EDTA Plasma 102 95% - 109% Cit plasma 108 106% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
CSF-1-R in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CSF-1-R protein in mouse serum, plasma, cell culture supernatants, tissue, and cellular extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Macrophage colony-stimulating factor 1 receptor, also known as CSF-1-R, Macrophage Colony-Stimulating factor receptor, or CD115, is a cell-surface membrane tyrosine kinase encoded by the CSF1R gene. CSF-1-R acts as a single-pass type 1 membrane receptor for colony stimulating factor 1 (CSF) and IL-34, and assists in the regulation in survival, proliferation, and differentiation of hematopoietic precursor cells. CSF-1-R has been found to be overexpressed in various cancers and on tumor-associated macrophages. CSF-1-R is also involved in the regulation of osteoclast proliferation, differentiation, and is necessary for normal bone development. Activated CSF-1-R mediates activation of the MAP kinases MAPK1/ERK2, MAPK3/ERK1, and several SRC family kinases.
Sensitivity in Sample Diluent NS: 0.063ng/mL. Sensitivity in 1X Cell Extraction Buffer PTR: 0.04 ng/mL.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CSF-1-R Capture Antibody 1 x 600µl 10X Mouse CSF-1-R Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse CSF-1-R Lyophilized Recombinant Protein 1 x 600µl Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C FMS
- CD 115
- CD115
see all -
Database links
- Entrez Gene: 12978 Mouse
- SwissProt: P09581 Mouse
- Unigene: 22574 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab240681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse CSF-1-R ELISA Kit (ab240681)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CSF-1-R standard curve in Sample Diluent NS.The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR.The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CSF-1-R in mouse serum, plasma (EDTA), plasma (heparin), and plasma (citrate) samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:3,200, plasma (EDTA) 1:3,200, plasma (heparin) 1:3,200, and plasma (citrate) 1:1,600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 6.8 µg/mL in serum, 6.9 µg/mL in plasma (EDTA), 6.8 µg/mL in plasma (heparin), and 3.9 µg/mL in plasma (citrate).
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Interpolated concentrations of native CSF-1-R in mouse spleen cell supernatant, liver cell supernatant, and RAW cell supernatant samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell supernatant 2.5%, liver cell supernatant 25%, and RAW cell supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 78.9 ng/mL in neat spleen cell supernatant, 13 ng/mL in neat liver cell supernatant, and 1,024 ng/mL in neat RAW cell supernatant.
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Interpolated concentrations of native CSF-1-R in mouse spleen cell extract and RAW 264.7 cell extract samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell extract 75 µg/mL and RAW264.7 cell extract 37.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Protocols
References
ab240681 has not yet been referenced specifically in any publications.
