Mouse CXCL1 ELISA Kit (ab213859)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse CXCL1 ELISA Kit
See all GRO alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 95.7pg/ml 5.84 6.1% 2 16 279pg/ml 12.56 4.5% 3 16 570pg/ml 36.48 6.4% Inter-assay Sample n Mean SD CV% 1 24 101.3pg/ml 7.29 7.2% 2 24 268pg/ml 22.78 8.5% 3 24 605pg/ml 41.14 6.8% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse CXCL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213859) is designed for the quantitative detection of Mouse CXCL1 in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R20-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CXCL1 amount of sample captured in plate.
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Notes
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO alpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. CXCL1 decreased the severity of multiple sclerosis and may offer a neuroprotective function.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse CXCL1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Mouse CXCL1 antibody 1 x 130µl Lyophilized recombinant Mouse CXCL1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
Database links
- Entrez Gene: 14825 Mouse
- SwissProt: P12850 Mouse
- Unigene: 21013 Mouse
Applications
Our Abpromise guarantee covers the use of ab213859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Mouse CXCL1 ELISA Kit (ab213859) Standard CurveMouse CXCL1 ELISA Kit (ab213859) Standard Curve
Protocols
References
ab213859 has not yet been referenced specifically in any publications.
