Overview

  • Product name

    Mouse CXCL1 ELISA Kit
    See all GRO alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 8 6.8%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 10.1%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.66 pg/ml
  • Range

    5.47 pg/ml - 350 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91 88% - 94%
    Cell culture extracts 95 95% - 96%
    Cell culture media 110 109% - 110%
    Heparin Plasma 79 72% - 90%
    EDTA Plasma 94 89% - 98%
    Citrate Plasma 94 92% - 97%

  • Assay time

    1h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow, Human
  • Product overview

    CXCL1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse CXCL1 protein in serum, plasma, cell culture supernatant, and cell extract samples.


    The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody. The capture antibody is incubated with the sample and then after washing, the detector antibody is added to the wells. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in 1X Cell Extraction Buffer PTR: 0.70 pg/mL
    Samples in Sample Diluent NS: 0.66 pg/mL

  • Notes

    CXCL1 is a member of the CXC family of chemokines. Chemokines play roles in normal and pathological processes including allergic responses, angiogenesis, inflammation, tumor growth and metastasis. Mouse CXCL2 and CXCL3 share 67% and 60% sequence homology with mouse CXCL1, respectively. Additionally, Rat CXCL1 is 89% homologous with mouse CXCL1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CXCL1 Capture Antibody 1 x 600µl
    10X Mouse CXCL1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT 1 x 30ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI 2 x 6ml
    Mouse CXCL1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • CXCL1
    • Cytokine-induced neutrophil chemoattractant 1
    • Fibroblast secretory protein
    • Fsp
    • Gro
    • Gro 1
    • Gro A
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO-alpha(6-73)
    • Gro1
    • Gro1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • GROA_HUMAN
    • Growth-regulated alpha protein
    • KC
    • KC chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • Melanoma growth stimulatory activity, alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • N51
    • NAP-3
    • NAP3
    • Neutrophil-activating protein 3
    • Platelet-derived growth factor-inducible protein KC
    • Scyb 1
    • Scyb1
    • Secretory protein N51
    • Small inducible cytokine subfamily B, member 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve comparison between mouse CXCL1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 386.84 pg/mL in neat serum, 399.45 pg/mL in neat plasma (citrate), 364.34 pg/mL in neat plasma (EDTA), and 505.80 pg/mL in neat plasma (heparin).

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 48.56 pg/mL in neat serum, 24.34 pg/mL in neat plasma (citrate), 23.67 pg/mL in neat plasma (EDTA), and 176.56 pg/mL in neat plasma (heparin).

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: NIH 3T3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 472.88 pg/mL in neat mouse NIH 3T3 cell culture supernatant.

  • The concentrations of CXCL1 were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 89.05 pg/mL in mouse NIH 3T3 cell extract sample.

Protocols

References

This product has been referenced in:

  • Fang C  et al. Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:3530-3536 (2018). Read more (PubMed: 29545879) »
  • Yan B  et al. HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice. Sci Rep 7:597 (2017). Read more (PubMed: 28377603) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216951.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up