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|Sample type||Average %||Range|
|Serum||120.1||102% - 128%|
|Plasma||134||118% - 150%|
|Cell culture media||135.5||124% - 144%|
Abcam’s CXCL15 mouse ELISA Kit (ab193720) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL15 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL15 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CXCL15 present in the samples. The wells are washed and biotinylated anti-mouse CXCL15 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CXCL15 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent D||1 x 15ml|
|800X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Biotinylated mouse CXCL15 detection antibody (lyophilized)||2 vials|
|mouse CXCL15 Standards (lyophilized)||2 vials|
|Pre-coated mouse CXCL15 Microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193720 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"