Mouse CXCL7 / PBP ELISA Kit (ab236713)
- Datasheet
- References
- Protocols
Overview
-
Product name
Mouse CXCL7 / PBP ELISA Kit
See all CXCL7/PBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.4% Inter-assay Sample n Mean SD CV% Serum 3 9.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.216 pg/ml -
Range
2.73 pg/ml - 175 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 91% - 97% Serum 101 83% - 118% Hep Plasma 108 106% - 109% EDTA Plasma 98 90% - 108% Cit plasma 87 83% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
CXCL7 / PBP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL7 / PBP protein in mouse serum, plasma and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
CXCL7 / PBP is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, and prostaglandin E2 secretion. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Rat and human share 73% and 66% sequence homology with mouse CXCL7 / PBP, respectively.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CXCL7 / PBP Capture Antibody 1 x 600µl 10X Mouse CXCL7 / PBP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CXCL7 / PBP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- B TG1
- Beta TG
- Beta thromboglobulin
see all -
Database links
- Entrez Gene: 57349 Mouse
Associated products
-
Alternative Versions
Images
-
Other - Mouse CXCL7 / PBP ELISA Kit (ab236713)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of mouse PPBP standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native PPBP in mouse serum, plasma and cell culture supernatant samples.The concentrations of PPBP were measured in duplicates, interpolated from the PPBP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:160K, plasma (citrate) 1:80K, plasma (heparin) 1:20K, plasma (EDTA) 1:20K and RAW 264.7 cell supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 23,028.15 ng/mL in serum, 1008.09 ng/mL in plasma (citrate) and 2447.06 ng/ml in plasma (heparin), 2828.45 ng/mL in plasma (EDTA).
-
Interpolated concentrations of native PPBP in mouse RAW 264.7 cell supernatant (100%).The concentrations of PPBP were measured in duplicate and interpolated from the PPBP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 41.45 pg/ml.
Protocols
References
ab236713 has not yet been referenced specifically in any publications.
