Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.216 pg/ml
- Range: 2.73 pg/ml - 175 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse CXCL7 / PBP ELISA Kit
See all CXCL7/PBP kits
Intra-assay Sample n Mean SD CV% Serum 8 3.4% Inter-assay Sample n Mean SD CV% Serum 3 9.3%
Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range2.73 pg/ml - 175 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 91% - 97% Serum 101 83% - 118% Hep Plasma 108 106% - 109% EDTA Plasma 98 90% - 108% Cit plasma 87 83% - 91%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse CXCL7 / PBP ELISA Kit (ab236713) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL7 / PBP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL7 / PBP with 0.216 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse CXCL7 / PBP Capture Antibody 1 x 600µl 10X Mouse CXCL7 / PBP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CXCL7 / PBP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
- Information by UniProt
- B TG1
- Beta TG
- Beta thromboglobulin
- Entrez Gene: 57349 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of PPBP were measured in duplicates, interpolated from the PPBP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:160K, plasma (citrate) 1:80K, plasma (heparin) 1:20K, plasma (EDTA) 1:20K and RAW 264.7 cell supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 23,028.15 ng/mL in serum, 1008.09 ng/mL in plasma (citrate) and 2447.06 ng/ml in plasma (heparin), 2828.45 ng/mL in plasma (EDTA).
The concentrations of PPBP were measured in duplicate and interpolated from the PPBP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 41.45 pg/ml.
To learn more about the advantages of recombinant antibodies see here.
ab236713 has not yet been referenced specifically in any publications.