Overview

  • Product name

    Mouse CXCL9 ELISA Kit
    See all CXCL9 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 7.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 7.5%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.4 pg/ml
  • Range

    6.25 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 133.7 119.4% - 144%
    Cell culture media 101.1 95.3% - 106%
    Heparin Plasma 87.9 87.8% - 88.1%
    EDTA Plasma 115.2 108.3% - 125.6%
    Citrate Plasma 104 95.9% - 109.2%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rabbit, Cow, Dog, Human, Pig
  • Product overview

    CXCL9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL9 protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples diluted in Sample Diluent NS – 1.4 pg/mL
    Samples diluted in Sample Diluent 25BS – 2.7 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 6.3 pg/mL

  • Notes

    MIG (Monokine Induced by Gamma interferon) is a small cytokine belonging to the CXC motif chemokine family, thus named CXC motif chemokine family ligand 9 (CXCL9). CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CXCL9 Capture Antibody 1 x 600µl
    10X Mouse CXCL9 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI 1 x 6ml
    Mouse CXCL9 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 9
    • chemokine (C-X-C motif) ligand 9
    • CMK
    • crg-10
    • CXCL9
    • CXCL9_HUMAN
    • gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • monokine induced by gamma interferon
    • monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab203364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse MIG (CSCL9) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MIG were measured in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted serum and plasma (citrate and EDTA) samples were pre-diluted to 50%. Note that 1X Diluted plasma (heparin) samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of MIG per mL of neat sample (mean +/- SD, n=2).

  • Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL mouse recombinant Interferon-gamma protein for 72 hours. The cell culture supernatant samples were collected. The concentrations of MIG were measured in the diluted supernatants in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were pre-diluted to 2%. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mL of neat sample (mean +/- SD, n=2).

  • Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL Mouse recombinant Interferon-gamma protein for 72 hours. The concentrations of MIG were measured in diluted extracts of these cells and of Mouse liver in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted Raw 264.7 cells extract samples were at 25 µg/mL. Note that 1X Diluted Mouse liver extract samples were at 500 µg/mL. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mg of total protein (mean +/- SD, n=2).

Protocols

References

ab203364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203364.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up