Overview

  • Product name

    Mouse Cystatin C ELISA Kit
    See all Cystatin C kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s mouse Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

    A Cystatin C specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cystatin C amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-mouse Cystatin C antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- mouse Cystatin C antibody 1 x 130µl
    Lyophilized recombinant Mouse Cystatin C standard 2 x 20ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificity

    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in disease

    Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.
    Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the cystatin family.
  • Post-translational
    modifications

    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ARMD11
    • bA218C14.4 (cystatin C)
    • Cst3
    • Cystatin-3
    • Cystatin-C
    • CYTC_HUMAN
    • Gamma-trace
    • Neuroendocrine basic polypeptide
    • Post-gamma-globulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119590.

Protocols

References

This product has been referenced in:

  • Nair AV  et al. Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo. ACS Nano 9:3641-53 (2015). Read more (PubMed: 25790730) »
  • De Chiara L  et al. Renal Cells from Spermatogonial Germline Stem Cells Protect against Kidney Injury. J Am Soc Nephrol N/A:N/A (2013). Functional Studies ; Mouse . Read more (PubMed: 24136918) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

Read More
Answer

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

Read More

Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

Read More
Answer

Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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