Product nameMouse Cytokine Antibody Array (Membrane, 40 Targets)
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Mouse
Abcam's Mouse Cytokine Antibody Array (ab211068) for use with serum, plasma, cell culture media, cell lysate, tissue lysate and other liquid sample types.
Targets: CD80 (B7-1), BAFF R, Betacellulin (BTC), C5a, CCL6, CD48 (SLAMF2), CD6, Chemerin, Clusterin, Lungkine (CXCL15), Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33 (IL-1 F11), IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2 (NGAL), LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV (CCN3), Osteoactivin (GPNMB), OX40 Ligand, P-Cadherin, Periostin, PLGF-2, Progranulin, Prostasin, Renin-1, Testican 3 (SPOCK3), TIM-1 (KIM-1), TRAIL (TNFSF10), Tryptase epsilon.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 1 vial 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab211068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab211068 has not yet been referenced specifically in any publications.