Mouse Decorin ELISA Kit (DCN) (ab155454)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Decorin ELISA Kit (DCN)
See all Decorin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% All < 10% Inter-assay Sample n Mean SD CV% All < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
> 68 %
Sample specific recovery Sample type Average % Range Serum 104.7 83% - 126% Plasma 93.91 74% - 119% Cell culture media 111.1 68% - 130% -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's Decorin (DCN) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse Decorin in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended as anticoagulants for use in this assay).
This assay employs an antibody specific for mouse Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse Decorin ELISA Kit (ab207618) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse Decorin 2 vials Decorin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Decorin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Bone proteoglycan II
- CSCD
- DCN
see all -
Database links
- Entrez Gene: 13179 Mouse
- SwissProt: P28654 Mouse
- Unigene: 56769 Mouse
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155454 has been referenced in 3 publications.
- Jiang R et al. Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Aβ amyloidosis. Acta Neuropathol Commun 10:96 (2022). PubMed: 35787306
- Chatterjee J et al. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia. Nat Commun 12:7122 (2021). PubMed: 34880260
- Gubbiotti MA et al. Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy. Matrix Biol 48:14-25 (2015). PubMed: 26344480