Mouse DPPIV ELISA Kit (CD26) (ab155455)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DPPIV ELISA Kit (CD26) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
> 68 %
Sample specific recovery Sample type Average % Range Serum 101.4 94% - 109% Plasma 88.06 77% - 97% Cell culture media 77.89 68% - 89% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's DPPIV (CD26) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse DPPIV (CD26) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for DPPIV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPPIV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPPIV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPPIV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse DPPIV 2 vials DPPIV Microplate (12 x 8 wells) 1 unit Recombinant Mouse DPPIV Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- Entrez Gene: 13482 Mouse
- SwissProt: P28843 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155455 has not yet been referenced specifically in any publications.