Mouse E-Cadherin ELISA Kit (CDH1) (ab100678)
- Datasheet
- References
- Protocols
Overview
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Product nameMouse E-Cadherin ELISA Kit (CDH1)
See all E Cadherin kits -
Detection methodColorimetric
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Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 6 pg/ml
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Range4.12 pg/ml - 3000 pg/ml
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Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 114.7 103% - 123% Serum 78.78 69% - 88% Plasma 88.93 76% - 95% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam’s E-Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E-Cadherin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse E-Cadherin coated on a 96- well plate. Standards and samples are pipetted into the wells and E-Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse E-Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E-Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Mouse E-Cadherin ELISA Kit (ab197751) from our SimpleStep ELISA® range.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse E-Cadherin 2 vials E Cadherin Microplate (12 x 8 wells) 1 unit Recombinant Mouse E-Cadherin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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RelevanceOne of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
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Alternative names
- Arc 1
- Cadherin 1
- cadherin 1 type 1 E-cadherin
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Database links
- Entrez Gene: 12550 Mouse
- SwissProt: P09803 Mouse
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab100678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
Protocols
References
ab100678 has not yet been referenced specifically in any publications.
