Overview

  • Product name
    Mouse E-Cadherin ELISA Kit (CDH1)
    See all E Cadherin kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 114.7 103% - 123%
    Serum 78.78 69% - 88%
    Plasma 88.93 76% - 95%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s E-Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E-Cadherin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse E-Cadherin coated on a 96- well plate. Standards and samples are pipetted into the wells and E-Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse E-Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E-Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse E-Cadherin ELISA Kit (ab197751) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse E-Cadherin 2 vials
    E Cadherin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse E-Cadherin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
  • Alternative names
    • Arc 1
    • Cadherin 1
    • cadherin 1 type 1 E-cadherin
    • Cadherin1
    • CAM 120/80
    • CD 234
    • CD324
    • CD324 antigen
    • CDH1
    • CDHE
    • ECAD
    • Epithelial cadherin
    • epithelial calcium dependant adhesion protein
    • LCAM
    • Liver cell adhesion molecule
    • UVO
    • Uvomorulin
    see all
  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab100678 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Represntative Standard Curve using ab200678

    • Typical Standard Curve
      Typical Standard Curve

      Represntative Standard Curve using ab200678

    Protocols

    References

    ab100678 has not yet been referenced specifically in any publications.

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