Overview

  • Product name

    Mouse E-Selectin ELISA Kit (CD62E)
    See all E-selectin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 2.57%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.13%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    8.55 pg/ml
  • Range

    23.4 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 84% - 89%
    Cell culture media 102 97% - 106%
    Hep Plasma 92 84% - 103%
    EDTA Plasma 94 90% - 100%
    Cit plasma 93 85% - 99%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig
  • Product overview

    Abcam’s E-Selectin (CD62E) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of E-Selectin protein mouse serum, citrate plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    E-Selectin is a cell-surface glycoprotein that plays a role in immune-adhesion. It is expressed by cytokine-stimulated endothelial cells, and mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. E-selectin is also thought to have a role in capillary morphogenesis. Its structural features include the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab201279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The 1X dilution for serum was 4%. The 1X dilution for EDTA plasma was 0.5%, and the 1X dilution for citrate and heparin plasma was 2%. Interpolated data values (mean +/- SD, n = 2) are graphed.

Protocols

References

This product has been referenced in:

  • Wang Y  et al. Cannabidiol attenuates alcohol-induced liver steatosis, metabolic dysregulation, inflammation and neutrophil-mediated injury. Sci Rep 7:12064 (2017). Read more (PubMed: 28935932) »
See 1 Publication for this product

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