Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.55 pg/ml
- Range: 23.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse E-Selectin ELISA Kit (CD62E)
See all E-selectin kits
Intra-assay Sample n Mean SD CV% Overall 8 2.57% Inter-assay Sample n Mean SD CV% Overall 3 4.13%
Sample typeCell culture supernatant, Serum, Cit plasma
Assay typeSandwich (quantitative)
Range23.4 pg/ml - 1500 pg/ml
Sample specific recovery Sample type Average % Range Serum 85 84% - 89% Cell culture media 102 97% - 106% Hep Plasma 92 84% - 103% EDTA Plasma 94 90% - 100% Cit plasma 93 85% - 99%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Goat, Cow, Pig
Mouse E-Selectin ELISA Kit (CD62E) (ab201279) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Selectin (CD62E) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Selectin (CD62E) with 8.55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
E-Selectin is a cell-surface glycoprotein that plays a role in immune-adhesion. It is expressed by cytokine-stimulated endothelial cells, and mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. E-selectin is also thought to have a role in capillary morphogenesis. Its structural features include the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse E-Selectin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse E-Selectin Capture Antibody (Lyophilized) 1 vial Mouse E-Selectin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
- Information by UniProt
- CD62 antigen-like family member E
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The 1X dilution for serum was 4%. The 1X dilution for EDTA plasma was 0.5%, and the 1X dilution for citrate and heparin plasma was 2%. Interpolated data values (mean +/- SD, n = 2) are graphed.
To learn more about the advantages of recombinant antibodies see here.
ab201279 has been referenced in 2 publications.
- Ye W et al. Lipoxin A4 Ameliorates Acute Pancreatitis-Associated Acute Lung Injury through the Antioxidative and Anti-Inflammatory Effects of the Nrf2 Pathway. Oxid Med Cell Longev 2019:2197017 (2019). PubMed: 31781326
- Wang Y et al. Cannabidiol attenuates alcohol-induced liver steatosis, metabolic dysregulation, inflammation and neutrophil-mediated injury. Sci Rep 7:12064 (2017). PubMed: 28935932