Overview

  • Product name

    Mouse EGF ELISA Kit
    See all EGF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 5 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 11%
  • Sample type

    Cell culture supernatant, Urine, Serum, Tissue Extracts, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.03 pg/ml
  • Range

    1.56 ng/ml - 150 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 97 92% - 102%
    Serum 103 101% - 105%
    Tissue Extracts 90 85% - 94%
    Cell culture media 97 93% - 101%
    EDTA Plasma 101 100% - 102%
    Citrate Plasma 114 101% - 120%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Product overview

    EGF in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of EGF protein in mouse serum, plasma, urine, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Mouse EGF precursor is a large 1189 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Mouse EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 1.05 pg/ml


    Samples diluted in 1X Cell Extraction Buffer PTR - 1.05 pg/ml


    Samples diluted in Sample Diluent 25BS - 1.03 pg/ml

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    10X Mouse EGF Capture Antibody 1 x 600µl
    10X Mouse EGF Detector Antibody 1 x 600µl
    Mouse EGF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab234560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:10,000 and kidney supernatant 1:4. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 600,860 pg/mL in neat urine and 384.1 pg/mL in neat kidney tissue supernatant.

  • The concentrations of EGF were measured in duplicate and interpolated from the EGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 90.16 pg/mL in mouse kidney tissue extract.

  • The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native EGF concentration was determined to be 7.456 pg/mL in neat serum, 63.90 pg/mL in neat plasma (citrate), and not detectable in neat plasma (EDTA). Native data is not shown.

  • Standard curve comparison between Mouse EGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity

Protocols

References

ab234560 has not yet been referenced specifically in any publications.

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