Mouse Eotaxin-1 ELISA Kit (ab100680)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameMouse Eotaxin-1 ELISA Kit
See all Eotaxin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 1 pg/ml
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Range1.02 pg/ml - 100 pg/ml
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Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.25 83% - 104% Serum 94.95 83% - 104% Plasma 92.54 84% - 103% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam’s Eotaxin-1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Eotaxin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Eotaxin-1 2 vials Eotaxin-1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse Eotaxin-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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FunctionIn response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsO-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- C-C motif chemokine 11
- CCL 11
- CCL11
see all -
Database links
- Entrez Gene: 20292 Mouse
- SwissProt: P48298 Mouse
- Unigene: 4686 Mouse
Applications
Our Abpromise guarantee covers the use of ab100680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab100680
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Typical Standard CurveRepresentative standard curve using ab100680
Protocols
References
This product has been referenced in:
- Ren X et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). Read more (PubMed: 23149934) »
- Ci X et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). Read more (PubMed: 22563373) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"