Overview

  • Product name
    Mouse Eotaxin-1 ELISA Kit
    See all Eotaxin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1.02 pg/ml - 100 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 83% - 104%
    Serum 94.95 83% - 104%
    Plasma 92.54 84% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Eotaxin-1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse Eotaxin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    80x HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Eotaxin-1 2 vials
    Eotaxin-1 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Eotaxin-1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • Chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil Chemotactic P
    • Eosinophil chemotactic protein
    • Eotaxin
    • Eotaxin 1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100680

  • Representative standard curve using ab100680

Protocols

References

This product has been referenced in:
  • Ren X  et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). Read more (PubMed: 23149934) »
  • Ci X  et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). Read more (PubMed: 22563373) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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