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    mouse-eotaxin-1-elisa-kit-ab100680.pdf

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Immunology Innate Immunity Chemokines Beta Chemokines (CC)
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Mouse Eotaxin-1 ELISA Kit (ab100680)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 1 pg/ml
  • Range: 1.02 pg/ml - 100 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Eotaxin-1 ELISA Kit
    See all Eotaxin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    1.02 pg/ml - 100 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 83% - 104%
    Serum 94.95 83% - 104%
    Plasma 92.54 84% - 103%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Eotaxin-1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse Eotaxin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    80x HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Eotaxin-1 2 vials
    Eotaxin-1 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Eotaxin-1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Function

    In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P51671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil chemotactic protein
    • Eotaxin
    • eotaxin-1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all
  • Database links

    • Entrez Gene: 20292 Mouse
    • SwissProt: P48298 Mouse
    • Unigene: 4686 Mouse

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100680

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100680

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab100680? Please let us know so that we can cite the reference in this datasheet.

    ab100680 has been referenced in 2 publications.

    • Ren X  et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). PubMed: 23149934
    • Ci X  et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). PubMed: 22563373

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