Overview

  • Product name

    Mouse Eotaxin-2 Matched Antibody Pair Kit
    See all Eotaxin 2 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    7.8 pg/ml - 500 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Eotaxin 2. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tip on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab206985) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse Eotaxin 2 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse Eotaxin 2 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse Eotaxin 2 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • Tissue specificity

    Activated monocytes and activated T lymphocytes.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C C motif chemokine 24
    • C-C motif chemokine 24
    • CCL24
    • CCL24_HUMAN
    • Chemokine CC Motif Ligand 24
    • CK beta 6
    • CK-beta-6
    • Ckb6
    • Eosinophil chemotactic protein 2
    • Eotaxin-2
    • MPIF 2
    • MPIF-2
    • MPIF2
    • Myeloid progenitor inhibitory factor 2
    • SCYA24
    • Small inducible cytokine A24
    • Small inducible cytokine subfamily A (Cys-Cys) member 24
    • Small-inducible cytokine A24
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab213460 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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