Mouse Erythropoitin ELISA Kit (EPO) (ab119593)


  • Product name
    Mouse Erythropoitin ELISA Kit (EPO)
    See all Erythropoietin (EPO) kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    46.9 pg/ml - 3000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse erythropoitin (EPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse erythropoitin (EPO) in cell culture supernatants, serum, plasma (heparin) and tissue homogenates. 

    A erythropoitin (EPO) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for erythropoitin (EPO) is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse erythropoitin (EPO) amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab119593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab119593



ab119593 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

ab119593: Erythropoietin

Good Excellent 5/5 (Ease of Use)
Sample: Mouse serum
Specification: EDTA treated mouse blood plasma
Erythropoietin levels were measured in mouse blood plasma samples collected during harvest in EDTA-containing syringes.
Samples were diluted 1:4.
The Erythropoietin mouse ELISA Kit works out fine and is easy to use.

Abcam user community

Verified customer

Submitted Apr 25 2017

The standard curve should look similar to the example provided on the online product datasheet for ab119593. Please note the chart uses log scale on both X and Y axes. Using a different scale will alter the shape of the curve.

Thank you for your reply.

The information we have on the datasheet indicates that the plate format is 12 strips making up the 96-well plate precoated with anti-mouse EPO antibody. Just disconnect and use the strips you need, and seal and rest...

Read More

Thank you for your inquiry and I apologize for the delay in this reply as I was awaiting more information from the lab.

The plate in the kit is divisible so you can use it at different times. However, we wouldn't recommend washing and reusing...

Read More

Thank you for your enquiry.

We do not have set expiration dates for our products. Mostkitsare stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instru...

Read More

Thank you for contacting us.

I am sorry but I could not find the lot number provided. Do you have the order number on which it was purchased?

If the product is stored as advised on the datasheet of this product:


Read More

Thank you for contacting Abcam.
I have talked to the supplier about this product and they say that the kit will cross react with rat samples, however they do not recommend using the kit with rat tissue.
Please let me know if there is anything...

Read More


Sign up