Mouse FAK (phospho Y397) peptide (ab40145)

Abreviews (1)Q&A (1)

Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Mouse

Specifications

Our Abpromise guarantee covers the use of ab40145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • FADK
    • FADK 1
    • FAK related non kinase polypeptide
    • FAK1
    • FAK1_HUMAN
    • Focal adhesion Kinase
    • Focal adhesion kinase 1
    • Focal adhesion kinase isoform FAK Del33
    • Focal adhesion kinase related nonkinase
    • FRNK
    • p125FAK
    • pp125FAK
    • PPP1R71
    • Protein phosphatase 1 regulatory subunit 71
    • Protein tyrosine kinase 2
    • Protein-tyrosine kinase 2
    • Ptk2
    • PTK2 protein tyrosine kinase 2
    see all
  • Function
    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity
    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain
    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization
    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt

Images

  • Blocking - Mouse FAK (phospho Y397) peptide (ab40145)
    Blocking - Mouse FAK (phospho Y397) peptide (ab40145)This image is courtesy of an anonymous Abreview

    To demonstrate the specificity of the phosphorylation site specific antibody (ab4803) a 1/150 dilution of anti human-FAK (phospho Y397) antibody was pre-incubated with 200 fold molar excess of peptide (ab40145, 1/15 dilution) for 12 hours rolling at 4°C.

    Human Tissue sections (kidney) were deparaffinized, rehydrated and antigen retrieval was performed using citrate buffer pH 6.0. Endogenous peroxidase was quenched using 3% hydrogenperoxide in methanol and blocking was performed. Primary antibody alone (positive control) or mixed with peptide (blocking control) or antibody diluent alone (negative control) was applied to tissue sections and incubated for 16 hours at 4°C. Detection was performed using a HRP detection system. Nuclei were counterstained using Hematoxylin.

  • Western blot - FAK (phospho Y397) antibody (ab39967)
    Western blot - FAK (phospho Y397) antibody (ab39967)

References

ab40145 has not yet been referenced specifically in any publications.

Publishing research using ab40145? Please let us know so that we can cite the reference in this datasheet.

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Peptide Competition Experiment

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Abreviews
Application
Immunohistochemistry
To demonstrate the specificity of the phosphorylation site specific antibody (ab4803) a 1/150 dilution of anti human-FAK (phospho Y397) antibody in DAKO AB diluent was pre-incubated with 200 fold molar excess of peptide (1/15 dilution) for 12h rolling at 4°C.
Human Tissue sections (kidney) were deparaffinized, rehydrated and antigen retrieval was performed using citrate buffer pH 6.0. Endogenous peroxidase was quenched using 3% hydrogenperoxide in methanol and blocking was performed with ultra V block (Thermo Scientific™). Primary antibody alone (positive control) or mixed with peptide (blocking control) or antibody diluent alone (negative control) was applied to tissue sections and incubated for 16h at 4°C. Detection was performed using Ultravision LP Detection System (Thermo Scientific™) with HRP. Nuclei were counterstained using Hematoxylin.
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Abcam user community

Verified customer

Submitted Mar 12 2014

Is there a peptide for use with this antibody as a positive control.

Thank you for contacting us.

We do offer theFAK peptide - phospho Y397. The product number is ab40145 and may be located at the following link:

https://www.abcam.com/FAK-peptide-phospho-Y397-ab40145.html

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