Overview

  • Product name
    Mouse Ferritin ELISA Kit (FTL)
    See all Ferritin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    3.163 ng/ml
  • Range
    12.5 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's Ferritin (FTL) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin (FTL) in mouse biological samples.


     


    In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-mouse Ferritin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Mouse Ferritin Calibrator (lyophilized) 1 vial
    Mouse Ferritin ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Alternative names
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Ferritin measured in biologicals showing quantity (ng) per mL of tested sample

  • Representative standard curve using ab157713 Ferritin Mouse ELISA kit

Protocols

References

This product has been referenced in:
  • Saitoh S  et al. 5-aminolevulinic acid (ALA) deficiency causes impaired glucose tolerance and insulin resistance coincident with an attenuation of mitochondrial function in aged mice. PLoS One 13:e0189593 (2018). ELISA ; Mouse . Read more (PubMed: 29364890) »
  • Folgueras AR  et al. Matriptase-2 deficiency protects from obesity by modulating iron homeostasis. Nat Commun 9:1350 (2018). Read more (PubMed: 29636509) »

See all 5 Publications for this product

Customer reviews and Q&As

Usage of ab157713 on cell lysate

Good Good 4/5 (Ease of Use)
Abreviews
The kit successfully detected ferritin in a mouse cell line.
Murine cell line was grown in a 6 well plate and washed with PBS. 250 uL/ well of either 50 mM NaoH or RIPA buffer, each containing protease inhibitor cocktail were added to promote cell lysis. The cell extract was collected in a tube, vortexed briefly and then used in the kit.
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Verified customer

Submitted Dec 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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