Overview

  • Product name

    Mouse Fetuin A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.846 ng/ml
  • Range

    3.125 ng/ml - 100 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 3.125ng/ml - 100ng/ml

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Fetuin A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A protein in mouse plasma, serum and urine.

  • Notes

    In this assay the Fetuin present in samples reacts with the anti-Fetuin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Fetuin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Fetuin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Fetuin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Fetuin in the test sample. The quantity of Fetuin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab205074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205074 Mouse Fetuin ELISA Kit.

Protocols

References

ab205074 has not yet been referenced specifically in any publications.

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