Overview

  • Product name
    Mouse Fibrinogen ELISA Kit
    See all Fibrinogen kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.63 µg/ml
  • Range
    0.63 µg/ml - 40 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 0m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Mouse Fibrinogen ELISA Kit (ab213478) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    1X Biotinylated Mouse Fibrinogen (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications
    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • FGA
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab108844 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent dilution.

    Images

    • Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Standard curve: mean of duplicates (+/- SD) with background reads subtracted
    • Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)

      Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.

    Protocols

    References

    This product has been referenced in:
    • de la Rosa X  et al. Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice. Stroke 45:1453-9 (2014). Read more (PubMed: 24676774) »

    See 1 Publication for this product

    Publishing research using ab108844? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    I just wanted to confirm that the Biotinylated Mouse Fibrinogen Antibody comes lyophilized , but after reconstitution with 4 ml of diluent will be a 4x stock solution.

    Yes, you will need to reconstitute the vial with 4 ml diluents and then dilute biotin solution 1:4 (4x) for use.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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