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    mouse-fibrinogen-elisa-kit-ab108844.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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Mouse Fibrinogen ELISA Kit (ab108844)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (2)

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Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
  • Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)

Key features and details

  • Sensitivity: 0.54 µg/ml
  • Range: 0.63 µg/ml - 20 µg/ml
  • Sample type: Plasma
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Fibrinogen ELISA Kit
    See all Fibrinogen kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.6%
  • Sample type

    Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.54 µg/ml
  • Range

    0.63 µg/ml - 20 µg/ml
  • Recovery

    99 %

  • Assay time

    3h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Mouse Fibrinogen ELISA Kit (ab213478) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    2X Biotinylated Mouse Fibrinogen (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 7ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • Database links

    • Entrez Gene: 14161 Mouse
    • Entrez Gene: 110135 Mouse
    • Entrez Gene: 99571 Mouse
    • SwissProt: Q8K0E8 Mouse
    • SwissProt: Q8VCM7 Mouse

    Associated products

    • SimpleStep ELISA kits

      • Mouse Fibrinogen ELISA Kit (ab213478)

    Images

    • Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Standard curve: mean of duplicates (+/- SD) with background reads subtracted
    • Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)
      Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)

      Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab108844? Please let us know so that we can cite the reference in this datasheet.

    ab108844 has been referenced in 2 publications.

    • Makhloufi C  et al. Assessment of Thrombotic and Bleeding Tendency in Two Mouse Models of Chronic Kidney Disease: Adenine-Diet and 5/6th Nephrectomy. TH Open 4:e66-e76 (2020). PubMed: 32309772
    • de la Rosa X  et al. Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice. Stroke 45:1453-9 (2014). PubMed: 24676774

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I just wanted to confirm that the Biotinylated Mouse Fibrinogen Antibody comes lyophilized , but after reconstitution with 4 ml of diluent will be a 4x stock solution.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 10 2013

    Answer

    Yes, you will need to reconstitute the vial with 4 ml diluents and then dilute biotin solution 1:4 (4x) for use.

    Read More

    Abcam Scientific Support

    Answered on Jun 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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