Overview

  • Product name

    Mouse Fibronectin ELISA Kit
    See all Fibronectin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 3 ng/ml
  • Range

    3.125 ng/ml - 200 ng/ml
  • Recovery

    101 %

  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Product overview

    Mouse Fibronectin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108849) is designed for the quantitative measurement of Fibronectin levels in plasma, serum, urine and cell culture supernatants.


    A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse Fibronectin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Fibronectin Microplate (12 x 8 well strips) 1 unit
    Fibronectin Standard (lyophilized) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Alternative names

    • CIG
    • Cold insoluble globulin
    • Cold-insoluble globulin
    • DKFZp686F10164
    • DKFZp686H0342
    • DKFZp686I1370
    • DKFZp686O13149
    • ED B
    • Fibronectin 1
    • FINC
    • FINC_HUMAN
    • FN
    • FN1
    • FNZ
    • GFND
    • GFND2
    • LETS
    • Migration stimulating factor
    • MSF
    • Ugl-Y3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Fibronectin measured in biological fluids showing quantity (ng) per mL of sample tested

  • Representative Standard Curve using ab108849

Protocols

References

This product has been referenced in:

  • Kasagi Y  et al. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation. Cancer Res 76:347-57 (2016). Read more (PubMed: 26744523) »
  • Jo DH  et al. Quantitative Proteomics Reveals ß2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability. Mol Cell Proteomics 15:1681-91 (2016). Read more (PubMed: 26969716) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Vielen Dank für Ihren Anruf heute Morgen.

Ich möchte hiermit nochmals bestätigen, was meine Kollegin heute Morgen am Telefon erwähnt hat. Dieses Kit ist ca. 1 Jahr haltbar, wenn es bei der angebenden Temperatur gelagert wird.

Unsere Garantie deckt alle unsere Produkte für sechs Monate ab. Wir empfehlen deshalb gerade Kits innerhalb diese Zeitraumes zu benutzen.

Bitte achten Sie auf die Angaben zur Lagerung im Protokoll, verschieden Komponenten haben oft unterschiedliche Lagerungstemperaturen.



Ich wünsche Ihnen viel Erfolg mit Ihrer Forschung und verbleibe

Read More

Answer

Gracias por contactarnos.

Nuestros productos se envían en bloques de hielo como medida simplemente preventiva, para mantenerlos a una temperatura constante (salvo que por especificaciones concretas haya que enviarlos a -20C, que en ese caso se envía en hielo seco).

En principio ninguno de los componentes del kit se ha debido degradar por el hecho de no haber llegado a 4C. Los anticuerpos, el estándar y el resto de soluciones son estables hasta una semana a temperatura ambiente, siempre que sea por un periodo de tiempo corto.

Lo que sí es imprescindible es que una vez recibido el kit, éste se guarde a 4C, y evitar ciclos de congelación / descongelación.

Así pues, yo os recomiendo que utilicéis el kit normalmente, y en caso de tener el más mínimo problema os animo a que nos contactéis de inmediato. Todos los productos de Abcam tienen una garantía de 6 meses, por lo que en caso de no funcionar como se especifica en la datasheet éste será reemplazado o reembolsado.

Espero haber resuelto vuestras dudas, en caso contrario, por favor, no dudéis en volver a contactarnos.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up