Key features and details
- Sensitivity: 3 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Fibronectin ELISA Kit
See all Fibronectin kits
Intra-assay Sample n Mean SD CV% Overall 4% Inter-assay Sample n Mean SD CV% Overall 9%
Sample typeCell culture supernatant, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity= 3 ng/ml
Range3.125 ng/ml - 200 ng/ml
Assay time3h 30m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Mouse Fibronectin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108849) is designed for the quantitative measurement of Fibronectin levels in plasma, serum, urine and cell culture supernatants.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Fibronectin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Fibronectin Microplate (12 x 8 well strips) 1 unit Fibronectin Standard (lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml
FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Involvement in diseaseGlomerulopathy with fibronectin deposits 2
Sequence similaritiesContains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains.
Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
- Cold insoluble globulin
- Cold-insoluble globulin
ab108849 has been referenced in 4 publications.
- Kasagi Y et al. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation. Cancer Res 76:347-57 (2016). PubMed: 26744523
- Jo DH et al. Quantitative Proteomics Reveals ß2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability. Mol Cell Proteomics 15:1681-91 (2016). PubMed: 26969716
- Brentnall M et al. Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function. J Cell Sci 127:2217-26 (2014). PubMed: 24610949
- Riopel MM et al. ß1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function. Lab Invest N/A:N/A (2012). PubMed: 23069938