Mouse Flt 3 Ligand ELISA Kit (ab113342)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameMouse Flt 3 Ligand ELISA Kit
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Detection methodColorimetric
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Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 15 pg/ml
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Range20 pg/ml - 5000 pg/ml
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Recovery
87 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.49 67% - 103% Serum 91.81 83% - 99% Plasma 77.99 69% - 85% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam’s Flt3 Ligand Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Flt3 Ligand in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse Flt 3 Ligand 2 vials Flt 3 Ligand Microplate (12 x 8 wells) 1 unit Recombinant Mouse Flt 3 Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- SwissProt: P49772 Mouse
Applications
Our Abpromise guarantee covers the use of ab113342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab113342
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Typical Standard CurveRepresentative standard curve using ab113342
Protocols
References
ab113342 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"