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Mouse Flt 3 Ligand ELISA Kit (ab113342)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 15 pg/ml
  • Range: 20 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Flt 3 Ligand ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    20 pg/ml - 5000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.49 67% - 103%
    Serum 91.81 83% - 99%
    Plasma 77.99 69% - 85%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Flt3 Ligand Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Flt3 Ligand in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse Flt 3 Ligand 2 vials
    Flt 3 Ligand Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Flt 3 Ligand Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
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  • Database links

    • SwissProt: P49772 Mouse

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab113342

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab113342

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab113342? Please let us know so that we can cite the reference in this datasheet.

      ab113342 has not yet been referenced specifically in any publications.

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