Mouse Fractalkine ELISA Kit (CX3CL1) (ab100683)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Mouse Fractalkine ELISA Kit (CX3CL1)
See all CX3CL1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.3 ng/ml -
Range
0.274 ng/ml - 200 ng/ml -
Recovery
78 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 74.84 67% - 81% Serum 74.56 67% - 82% Plasma 84.97 68% - 95% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s CX3CL1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CX3CL1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse CX3CL1 coated on a 96- well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse CX3CL1 (lyophilized) 2 vials CX3CL1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CX3CL1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. -
Alternative names
- A 152E5.2
- AB030188
- ABCD 3
see all -
Database links
- Entrez Gene: 20312 Mouse
- SwissProt: O35188 Mouse
Applications
Our Abpromise guarantee covers the use of ab100683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab100683
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Typical Standard CurveRepresentative standard curve using ab100683
Protocols
References
This product has been referenced in:
- Pappritz K et al. Cardiac (myo)fibroblasts modulate the migration of monocyte subsets. Sci Rep 8:5575 (2018). ELISA ; Mouse . Read more (PubMed: 29615815) »
