Mouse Fractalkine ELISA Kit (CX3CL1) (ab100683)

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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.3 ng/ml
  • Range: 0.274 ng/ml - 200 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Fractalkine ELISA Kit (CX3CL1)
    See all CX3CL1 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 0.3 ng/ml

  • Range

    0.274 ng/ml - 200 ng/ml

  • Recovery

    78 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.84 67% - 81%
    Serum 74.56 67% - 82%
    Plasma 84.97 68% - 95%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s CX3CL1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CX3CL1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse CX3CL1 coated on a 96- well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    160X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Mouse CX3CL1 (lyophilized) 2 vials
    CX3CL1 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse CX3CL1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.

  • Alternative names

    • A 152E5.2
    • AB030188
    • ABCD 3
    • ABCD3
    • C X3 C motif chemokine 1
    • C3Xkine
    • Chemokine (C X3 C motif) ligand 1
    • Chemokine C X3 C motif ligand 1
    • Chemokine CX3C Motif Ligand 1
    • CX3C membrane anchored chemokine
    • CXC3
    • CXC3C
    • FKN
    • Fractalkine
    • Neurotactin
    • NTN
    • NTT
    • SCYD 1
    • SCYD1
    • Small inducible cytokine D1
    • Small inducible cytokine subfamily D (Cys X3 Cys), member 1
    • Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • Small inducible cytokine subfamily D member 1
    see all

  • Database links

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab100683

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab100683

      References (1)

      Publishing research using ab100683? Please let us know so that we can cite the reference in this datasheet.

      ab100683 has been referenced in 1 publication.

      • Pappritz K  et al. Cardiac (myo)fibroblasts modulate the migration of monocyte subsets. Sci Rep 8:5575 (2018). ELISA ; Mouse . PubMed: 29615815

      Customer reviews and Q&As

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      Question


      Inquiry: Hi, Regarding product ab100683, the anti-Mouse CX3CL1 Elisa, is the 96 well plate composed of individual strips which can be removed, or are all 96 wells inseparable?

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      Answer

      Thank you for contacting us.
      The plate that comes in this kit is 12 strips with 8 well apiece.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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