Key features and details
- Range: 2.5 ng/ml - 500 ng/ml
- Sample type: Cit plasma, Other biological fluids
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Product nameMouse FX ELISA kit (total FX antigen)
See all Factor X kits
Sample typeOther biological fluids, Cit plasma
Range2.5 ng/ml - 500 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse FX ELISA kit (total FX antigen) (ab272779) is for the quantitative determination of total Factor X and Xa in biological fluids.
Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with Streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total Factor X in the sample.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Mouse FX Primary Antibody Lyophilized Vial 1 vial Mouse FX ELISA Plate 1 unit Mouse FX Standard Lyophilized Vial 1 vial Streptavidin-HRP Secondary Reagent 1 vial TMB Substrate 1 x 10ml
FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Activated factor Xa heavy chain
- Coagulation factor X
Our Abpromise guarantee covers the use of ab272779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab272779 has not yet been referenced specifically in any publications.