Key features and details
- Range: 0.01 ng/ml - 10 ng/ml
- Sample type: Cit plasma, EDTA Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Product nameMouse FXII ELISA kit (total FXII antigen)
See all Factor XII kits
Intra-assay Sample n Mean SD CV% Sample 1 20 0.055ng/ml 6.25% Sample 2 20 0.29ng/ml 2.88% Sample 3 20 0.825ng/ml 1.71% Inter-assay Sample n Mean SD CV% Sample 1 10 0.238ng/ml 9.04% Sample 2 10 0.725ng/ml 5.52% Sample 3 10 2.04ng/ml 6.19%
Sample typeEDTA Plasma, Cit plasma
Range0.01 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Spike 98 94% - 102%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse FXII ELISA kit (total FXII antigen) (ab272776) is intended for the quantitative determination of total Factor XII antigen in mouse plasma.
Mouse Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti-mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Mouse FXII Primary Antibody Lyophilized Vial 1 vial FXII ELISA Plate 1 unit Mouse FXII Standard Lyophilized Vial 1 vial Streptavidin-HRP Secondary Reagent 1 vial TMB Substrate 1 x 10ml
FunctionFactor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Involvement in diseaseDefects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsFactor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
- Information by UniProt
- Factor XII
- Beta factor XIIa part 1
- Beta factor XIIa part 2
ab272776 has not yet been referenced specifically in any publications.