Overview

  • Product name

    Mouse G-CSF ELISA Kit (CSF3)
    See all G-CSF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    RAW 264.7 5 5.7%
    Inter-assay
    Sample n Mean SD CV%
    RAW 264.7 3 11.9%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.4 pg/ml
  • Range

    12.5 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97.2 86.2% - 106%
    Cell culture media 103.5 98% - 106.5%
    Cit plasma 105.2 95.1% - 116.9%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig
  • Product overview

    Abcam’s G-CSF (CSF3) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of G-CSF protein in mouse serum, plasma and cell culture supernatant samples.
    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse G-CSF Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse G-CSF Capture Antibody (Lyophilized) 1 vial
    Mouse G-CSF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
  • Alternative names

    • C17orf33
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3OS
    • Csfg
    • Filgrastim
    • GCSA
    • GCSF
    • Granulocyte colony stimulating fact
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • MGI IG
    • OTTMUSP00000006785
    • Pluripoietin
    • RP23-395E10.4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab197743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Cell culture supernatant samples was measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.

Protocols

References

ab197743 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197743.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up