Mouse Galectin 3 ELISA Kit (ab113343)

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Overview

  • Product name

    Mouse Galectin 3 ELISA Kit
    See all Galectin 3 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 6 pg/ml

  • Range

    8.19 pg/ml - 2000 pg/ml

  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124.3 116% - 131%
    Serum 111.8 101% - 120%
    Plasma 99.08 90% - 107%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Galectin 3 2 vials
    Galectin 3 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Galectin 3 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain.

  • Cellular localization

    Cytoplasmic, Nuclear and Secreted

  • Alternative names

    • 35 kDa lectin
    • Carbohydrate-binding protein 35
    • CBP 35
    • Gal-3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin-3
    • GALIG
    • IgE-binding protein
    • L-31
    • Laminin-binding protein
    • Lectin L-29
    • lectin, galactoside-binding, soluble, 3
    • LGALS2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab113343 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab113343

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab113343

    Protocols

    References

    ab113343 has not yet been referenced specifically in any publications.

    Publishing research using ab113343? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I am using tissue homogenate as my sample for use with this kit. Maybe homogenate has not been tested before but there is no inidation as to which Diluent to use (A or B) with this type of sample.
    1. What is the difference between diluent A and B?
    2. Which diluent do you recommend for use with tissue homogenate?

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    Answer

    While this ELISA kit was neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test these kits with tissue lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the experimenter empirically.

    Concentration of the original lysate should ideally be at least 1 mg/ml protein, preferably more, to achieve 50-500 ug/ml after dilution. Please note this range should just be used as a starting point and the optimal concentration would need to be determined empirically.


    In general, we recommend your lysis buffer meet the following specifications:
    A) has relatively low salt content (700 mM or less)
    B) does not contain sodium azide
    C) does not contain >0.1% SDS
    D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)



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    Question


    We need a Galectin-3 ELISA kit specific for mouse and does not cross react with human at all. Did test the cross activity on this kit for human?

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    Answer

    Thank you for the clarification. This kit shows 10% reactivity with human. Please let me know if you have any further questions.

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