Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Galectin 3 ELISA Kit
See all Galectin 3 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 6 pg/ml
Range8.19 pg/ml - 2000 pg/ml
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 124.3 116% - 131% Serum 111.8 101% - 120% Plasma 99.08 90% - 107%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Galectin 3 2 vials Galectin 3 Microplate (12 x 8 wells) 1 unit Recombinant Mouse Galectin 3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain.
Cellular localizationCytoplasmic, Nuclear and Secreted
- 35 kDa lectin
- Carbohydrate-binding protein 35
- CBP 35
ab113343 has not yet been referenced specifically in any publications.