Overview

  • Product name

    Mouse Galectin 3 ELISA Kit
    See all Galectin 3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.9%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.4 pg/ml
  • Range

    7.81 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 96 94% - 98%
    Serum 107 105% - 108%
    Cell culture media 94 92% - 97%
    Hep Plasma 96 94% - 98%
    EDTA Plasma 111 107% - 115%
    Cit plasma 108 104% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Pig
  • Product overview

    Abcam’s Galectin-3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Galectin-3 protein in mouse serum, plasma, urine, and cell culture media.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is in the Galectin family of carbohydrate-binding proteins and is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Galectin-3 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPR 1 x 6ml
    Mouse Galectin-3 Capture Antibody (lyophilized) 1 vial
    Mouse Galectin-3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain.
  • Cellular localization

    Cytoplasmic, Nuclear and Secreted
  • Alternative names

    • 35 kDa lectin
    • Carbohydrate-binding protein 35
    • CBP 35
    • Gal-3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin-3
    • GALIG
    • IgE-binding protein
    • L-31
    • Laminin-binding protein
    • Lectin L-29
    • lectin, galactoside-binding, soluble, 3
    • LGALS2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab203369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 11.3 ng/mL in mouse serum, 13.2 ng/mL in mouse plasma (Citrate), and 12.9 ng/mL in mouse plasma (EDTA).

  • J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Galectin-3 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the Galectin-3 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 14 ng/mL unstimulated, 38.9 ng/mL stimulated, and undetectable in media.

Protocols

References

ab203369 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

ab203369 contains a RabMAb antibody pair where the immunogen for each RabMAb is full-length mature mouse Galectin-3. We unfortunately haven't epitope mapping.
The standard protein is E.coli expressed full length recombinant mouse Galectin-3 therefore, we would expect that oligomers could be detected however we cannot say definitively. Ideally purified homo-oligomers and hetero-oligomers could be obtained and tested in the kit to determine the answer.

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