Product nameMouse Galectin-3 Matched Antibody Pair Kit
See all Galectin 3 kits
Assay typeELISA set
Range3.9 pg/ml - 250 pg/ml
Species reactivityReacts with: Mouse
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Galectin-3. Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab203369) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
The capture antibody is glycerol free.
The detector antibody contains glycerol.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Mouse Galectin-3 Capture Antibody 1 x 100µg 1 x 20µg Mouse Galectin-3 Detector Antibody 1 x 25µg 1 x 5µg Mouse Galectin-3 Lyophilized Protein 1 vial 1 vial
FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
- Information by UniProt
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
Our Abpromise guarantee covers the use of ab214481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab214481 has not yet been referenced specifically in any publications.