Overview

  • Product name
    Mouse Galectin-7 ELISA Kit (GAL7)
    See all Galectin 7 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 65 pg/ml
  • Range
    0.066 ng/ml - 16 ng/ml
  • Recovery

    > 77 %

    Sample specific recovery
    Sample type Average % Range
    Serum 141.4 133% - 149%
    Plasma 107.3 77% - 135%
    Cell culture media 139 118% - 148%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's Galectin-7 (GAL7) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse Galectin-7 (GAL7) in serum, plasma (recommended using heparin and citrate as an anticoagulant), and cell culture supernatants.


    This assay employs an antibody specific for Galectin-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Galectin-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse Galectin-7 ELISA Kit (ab206988) from our SimpleStep ELISA® range.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    160X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-mouse Galectin-7 2 vials
    Galectin-7 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Galectin-7 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis.
  • Alternative names
    • GAL7
    • HKL14
    • Human keratinocyte lectin 14
    • Lectin galactoside binding soluble 7
    • LGALS7
    • LGALS7B
    • MGC151215
    • MGC151217
    • OTTMUSP00000025970
    • OTTMUSP00000038956
    • p53 induced gene 1 protein
    • PI7
    • PIG1
    • TP53I1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab155456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155456

Protocols

References

ab155456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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