Overview

  • Product name

    Mouse GAS6 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    20.48 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.6 68% - 81%
    Serum 124.6 110% - 142%
    Plasma 120.4 109% - 130%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam's GAS6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse GAS6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse GAS6 coated on a 96-well plate. Standards and samples are pipetted into the wells and GAS6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GAS6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GAS6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155447 -  Assay Diluent A.

  • Representative standard curve using ab155447 - Assay Diluent B.

Protocols

References

ab155447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

GAS6 EXPRESSION IN MOUSE PERICYTES

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Easy to use, worked very well. Would use again.

Abcam user community

Verified customer

Submitted Apr 05 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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