Mouse GDF-15 ELISA Kit (ab216947)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse GDF-15 ELISA Kit
See all GDF15 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% EDTA plasma 8 5% Inter-assay Sample n Mean SD CV% EDTA plasma 3 5.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
22.2 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 76 70% - 82% Cell culture media 89 84% - 93% Hep Plasma 83 77% - 91% EDTA Plasma 82 81% - 84% Cit plasma 85 83% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
GDF-15 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse GDF-15 protein in serum, plasma, and cell culture supernatants.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
The cytokine growth differentiation factor 15 (GDF-15) is one of 15 growth/differentiation factors (GDF-1 to GDF-15), all of which are members of the BMP family of TGF-beta superfamily proteins. These growth/differentiation factors are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. Specifically, mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 158 aa glycosylated prodomain (aa 31-188) and a 115 aa mature region (aa 189-303). Over aa sequence 189-303, mouse GDF-15 shares 97% and 68% aa identity with rat and human GDF-15, respectively.
In mice, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes. It is also expressed in the epithelium of the small intestine and mammary gland. GDF proteins have a critical role during embryonic development, particularly in the skeletal, nervous, and muscular systems.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse GDF-15 Capture Antibody 1 x 600µl 10X Mouse GDF-15 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse GDF-15 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GDF 15
- GDF-15
- Gdf15
see all -
Database links
- Entrez Gene: 23886 Mouse
- SwissProt: Q9Z0J7 Mouse
- Unigene: 31325 Mouse
Associated products
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ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse GDF-15 ELISA Kit (ab216947)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse GDF-15 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked GDF-15 in mouse serum and plasma samples.The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 4,030 pg/mL in neat serum, 4,261 pg/mL in neat plasma (citrate), 4,385 pg/mL in neat plasma (EDTA), and 3,981 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native GDF-15 levels in mouse serum and plasma samples.The concentrations of GDF-15 were measured in duplicate, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 837.9 pg/mL in neat serum, 895.2 pg/mL in neat plasma (citrate), 1,079.6 pg/mL in neat plasma (EDTA), and 884.6 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native GDF-15 levels in pregnant mouse serum, mouse L929 and lung culture media samples.The concentrations of GDF-15 were measured in duplicate, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant mouse serum 50%, L929 culture media 100%, and lung culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 3,268 pg/mL in neat pregnant mouse serum, 2,223 pg/mL in L929 culture media, and 3,665 pg/mL in lung culture media.
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Interpolated concentrations of native GDF-15 in unstimulated and stimulated L929 and J774A.1 culture media samples.Samples were loaded neat (100%). The concentrations of GDF-15 were measured in duplicates and interpolated from the GDF-15 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be below the limit of detection in unstimulated L929 culture media, 2,246 pg/mL in stimulated L929 culture media, 193 pg/mL in unstimulated J774A.1 culture media, and 583 pg/mL in stimulated J774A.1 culture media. L929 cells were grown for 72 hours in MEM media containing 10% fetal horse serum and 100 µg/mL Kanamycin (unstimulated) plus 1.5% PHA and 10 ng/mL PMA (stimulated). J774A.1 cells were grown for 72 hours in HGDMEM media containing 10% fetal bovine serum and 100 µg/mL Kanamycin (unstimulated) plus 1.5% PHA and 10 ng/mL PMA (stimulated).
Protocols
References
ab216947 has not yet been referenced specifically in any publications.
