Overview

  • Product name

    Mouse GDH ELISA Kit
    See all H6PD/GDH kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 3.3%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    60 pg/ml
  • Range

    0.313 ng/ml - 30 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 116 108% - 128%
    Cell culture extracts 94 90% - 98%
    Tissue Extracts 107 102% - 112%
    Hep Plasma 98 92% - 102%
    Cit plasma 89 87% - 93%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    GDH in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GDH protein in mouse cell and tissue extracts, serum, and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Mitochondrial glutamate dehydrogenase (GDH) is an enzyme that converts L-glutamate into alpha-ketoglutarate. GDH plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse GDH Capture Antibody 1 x 600µl
    10X Mouse GDH Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    Mouse GDH Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
  • Tissue specificity

    Present in most tissues examined, strongest in liver.
  • Involvement in disease

    Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • Sequence similarities

    In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
    In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
  • Cellular localization

    Endoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen.
  • Information by UniProt
  • Alternative names

    • 6 phosphogluconolactonase
    • 6-phosphogluconolactonase
    • 6PGL
    • CORTRD1
    • G6PD, H form
    • G6PDH
    • G6PE_HUMAN
    • GDH
    • GDH/6PGL endoplasmic bifunctional protein
    • Glucose 1 dehydrogenase
    • Glucose 6 phosphate dehydrogenase, salivary
    • Glucose dehydrogenase
    • Glucose dehyrogenase
    • Gpd1
    • H6pd
    • Hexose 6 phosphate dehydrogenase
    • Hexose 6 phosphate dehydrogenase (glucose 1 dehydrogenase)
    • Hexose-6-phosphate dehydrogenase
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab246532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The GDH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The GDH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native GDH in mouse NIH/3T3 cell extract based on a 250 μg/mL extract load, mouse liver tissue extract based on a 20 μg/mL extract load, and mouse brain tissue extract based on a 50 μg/mL extract load. The concentrations of GDH were measured in duplicate and interpolated from the GDH standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDH concentration was determined to be 1.940 ng/mL in NIH/3T3 cell extract, 8.822 ng/mL in liver tissue extract, and 5.428 ng/mL in brain tissue extract.

  • Other species reactivity was determined by measuring liver tissue extract samples of various species, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations in ng of GDH protein per mg of extract. The mean GDH concentration was determined to be 811.1 ng/mg in mouse liver extract, 762.4 ng/mg in rat liver extract, and 163.0 ng/mg in human liver extract.

Protocols

References

ab246532 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab246532.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up