For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    mouse-gdnf-elisa-kit-ab171178.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Developmental proteins ELISA kits
Share by email

Mouse GDNF ELISA Kit (ab171178)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Typical standard curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    You may also be interested in

    Protein
    Recombinant mouse GDNF protein (ab56286)

    View more associated products

    Overview

    • Product name

      Mouse GDNF ELISA Kit
      See all GDNF kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      31.2 pg/ml - 2000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Abcam’s mouse GDNF in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse GDNF in cell culture supernatants, serum and plasma (heparin, EDTA). 


      A GDNF specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for GDNF is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse GDNF amount of sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-mouse GDNF antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-mouse GDNF antibody 1 x 130µl
      Lyophilized recombinant mouse GDNF standard 2 x 10ng
      Plate Seal 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Developmental proteins ELISA kits
    • Relevance

      Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement.
    • Cellular localization

      Secreted
    • Alternative names

      • Astrocyte derived trophic factor
      • ATF
      • ATF1
      • ATF2
      • Glial cell line derived neurotrophic factor
      • HFB1 GDNF
      • hGDNF
      • HSCR3
      see all
    • Database links

      • Entrez Gene: 14573 Mouse
      • SwissProt: P48540 Mouse
      • Unigene: 4679 Mouse

      Associated products

        Images

        • Typical standard curve
          Typical standard curve

          Representative standard curve using ab171178.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (3)

        Publishing research using ab171178? Please let us know so that we can cite the reference in this datasheet.

        ab171178 has been referenced in 3 publications.

        • Franco-Robles E & López MG Agavins Increase Neurotrophic Factors and Decrease Oxidative Stress in the Brains of High-Fat Diet-Induced Obese Mice. Molecules 21:N/A (2016). PubMed: 27490526
        • Wei X  et al. GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties. Sci Rep 6:36779 (2016). PubMed: 27827452
        • Masvekar RR  et al. Morphine Enhances HIV-1SF162-Mediated Neuron Death and Delays Recovery of Injured Neurites. PLoS One 9:e100196 (2014). PubMed: 24949623

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        1-2 of 2 Abreviews or Q&A

        good kit

        Excellent Excellent 5/5 (Ease of Use)
        Abreviews
        Abreviews
        I used cell media for the kit, and it worked really well.

        Abcam user community

        Verified customer

        Submitted Feb 09 2017

        Question

        - Can the phenol red within the cell medium interfere with the measurement at 450nm?
        - Can she use the cellular surnatant without any pre-treatment? She found an article where it was describe a pre-treatment to acidify the sample.

        Read More

        Abcam community

        Verified customer

        Asked on May 19 2014

        Answer


        The phenol red within the cell medium should not interfere with the measurement at 450nm but we would suggest running a media control just to make sure which can also act as your negative control.

        With regards to the sample preparation, no additional pre-treatment of the customers samples is necessary when using cell supernatant however, it is true that acidifying the samples can enhance the activation of GDNF so if you wish to do this, you can but please remember to add alkali to neutralise the samples.

        Read More

        Elisa Thomas

        Abcam Scientific Support

        Answered on May 19 2014

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

        Get resources and offers direct to your inbox Sign up
        A-Z by research area
        • Cancer
        • Cardiovascular
        • Cell biology
        • Developmental biology
        • Epigenetics & Nuclear signaling
        • Immunology
        • Metabolism
        • Microbiology
        • Neuroscience
        • Signal transduction
        • Stem cells
        A-Z by product type
        • Primary antibodies
        • Secondary antibodies
        • Biochemicals
        • Isotype controls
        • Flow cytometry multi-color selector
        • Kits
        • Loading controls
        • Lysates
        • Peptides
        • Proteins
        • Slides
        • Tags and cell markers
        • Tools & Reagents
        Help & support
        • Support
        • Make an Inquiry
        • Protocols & troubleshooting
        • Placing an order
        • RabMAb products
        • Biochemical product FAQs
        • Training
        • Browse by Target
        Company
        • Corporate site
        • Investor relations
        • Company news
        • Careers
        • About us
        • Blog
        Events
        • Tradeshows
        • Conferences
        International websites
        • abcam.cn
        • abcam.co.jp

        Join with us

        • LinkedIn
        • facebook
        • Twitter
        • YouTube
        • Terms of sale
        • Website terms of use
        • Cookie policy
        • Privacy policy
        • Legal
        • Modern slavery statement
        © 1998-2021 Abcam plc. All rights reserved.