Overview

  • Product name
    Mouse GDNF ELISA Kit
    See all GDNF kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse GDNF in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse GDNF in cell culture supernatants, serum and plasma (heparin, EDTA). 

    A GDNF specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for GDNF is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse GDNF amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse GDNF antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-mouse GDNF antibody 1 x 130µl
    Lyophilized recombinant mouse GDNF standard 2 x 10ng
    Plate Seal 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Relevance
    Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement.
  • Cellular localization
    Secreted
  • Alternative names
    • Astrocyte derived trophic factor
    • ATF
    • ATF1
    • ATF2
    • Glial cell line derived neurotrophic factor
    • HFB1 GDNF
    • hGDNF
    • HSCR3
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab171178 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical standard curve
      Typical standard curve

    Protocols

    References

    This product has been referenced in:
    • Masvekar RR  et al. Morphine Enhances HIV-1SF162-Mediated Neuron Death and Delays Recovery of Injured Neurites. PLoS One 9:e100196 (2014). Read more (PubMed: 24949623) »

    See 1 Publication for this product

    Publishing research using ab171178? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    good kit

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    I used cell media for the kit, and it worked really well.
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2017

    - Can the phenol red within the cell medium interfere with the measurement at 450nm?
    - Can she use the cellular surnatant without any pre-treatment? She found an article where it was describe a pre-treatment to acidify the sample.


    The phenol red within the cell medium should not interfere with the measurement at 450nm but we would suggest running a media control just to make sure which can also act as your negative control.

    With regards to the sample preparation, n...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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