- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
AMPA selective glutamate receptor 1
AMPA-selective glutamate receptor 1
Glutamate receptor 1
Glutamate receptor ionotropic
Glutamate receptor ionotropic AMPA 1
Glutamate receptor, ionotropic, AMPA 1
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
Widely expressed in brain.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Western blot - Mouse Glutamate Receptor 1 (AMPA subtype) (phospho T840) peptide (ab12399)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
ab12108 detects 2 bands over 100kDa by Western Blot. Both of these bands are blocked by the immunizing phospho peptide (lane 4), but not by the non-modified peptide (lane 5). However the immunoprecipitation data (below) demonstrates that the upper band (at ~120kDa) is unlikely to be GluR1 and therefore is an unknown protein.
has not yet been referenced specifically in any publications.
Publishing research using ab12399? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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