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    mouse-gm-csf-elisa-kit-ab46078.pdf

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Immunology Innate Immunity Cytokines CSFs
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Mouse GM-CSF ELISA Kit (ab46078)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 9 pg/ml
    • Range: 15.6 pg/ml - 500 pg/ml
    • Sample type: Cell culture supernatant, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

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    Recombinant mouse GM-CSF protein (Active) (ab9742)

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    Overview

    • Product name

      Mouse GM-CSF ELISA Kit
      See all GM-CSF kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      A 8 279.2pg/ml 14.7 5.2%
      B 8 62.5pg/ml 2.52 4%
      Inter-assay
      Sample n Mean SD CV%
      A 7 271.9pg/ml 10.8 3.9%
      B 7 65.4pg/ml 4.3 6.5%
    • Sample type

      Cell culture supernatant, Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 9 pg/ml
    • Range

      15.6 pg/ml - 500 pg/ml
    • Assay time

      3h 45m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Abcam’s GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.


      A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.


      This kit will recognize both endogenous and recombinant mouse GM-CSF.


      Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests 2 x 96 tests
      10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
      200X Wash Buffer White 1 x 10ml 2 x 10ml
      Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
      Biotinylated anti-GM-CSF Red 1 x 400µl 2 x 400µl
      Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
      GM-CSF Microplate (12 x 8 well strips) 1 unit 2 units
      GM-CSF Standard (Lyophilized) Yellow 2 vials 4 vials
      HRP Diluent Red 1 x 23ml 1 x 23ml
      Stop Reagent Black 1 x 11ml 2 x 11ml
      Streptavidin-HRP 2 x 5µl 4 x 5µl
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • CSFs
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Growth factors and hormones ELISA kits
    • Function

      Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similarities

      Belongs to the GM-CSF family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P04141 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Colony stimulating factor
      • Colony Stimulating Factor 2
      • Colony stimulating factor 2 (granulocyte-macrophage)
      • Colony-stimulating factor
      • CSF
      • CSF 2
      • CSF2
      • CSF2_HUMAN
      • GM-CSF
      • GMCSF
      • Granulocyte Macrophage Colony Stimulating Factor
      • Granulocyte-macrophage colony-stimulating factor
      • MGC131935
      • MGC138897
      • MGI1GM
      • Molgramostin
      • Pluripoietin-a
      • Sargramostim
      see all
    • Database links

      • Entrez Gene: 12981 Mouse
      • SwissProt: P01587 Mouse
      • Unigene: 4922 Mouse

      Associated products

      • SimpleStep ELISA kits

        • Mouse GM-CSF ELISA Kit (CSF2) (ab201276)

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab46078

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab46078? Please let us know so that we can cite the reference in this datasheet.

      ab46078 has been referenced in 1 publication.

      • Wang J  et al. Mediating macrophage immunity with wogonin in mice with vascular inflammation. Mol Med Rep 16:8434-8440 (2017). PubMed: 28983597

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