Mouse GM-CSF ELISA Kit (ab46078)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Mouse GM-CSF ELISA Kit
See all GM-CSF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 8 279.2pg/ml 14.7 5.2% B 8 62.5pg/ml 2.52 4% Inter-assay Sample n Mean SD CV% A 7 271.9pg/ml 10.8 3.9% B 7 65.4pg/ml 4.3 6.5% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 9 pg/ml -
Range
15.6 pg/ml - 500 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.
This kit will recognize both endogenous and recombinant mouse GM-CSF.
Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-GM-CSF Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml GM-CSF Microplate (12 x 8 well strips) 1 unit 2 units GM-CSF Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 23ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
Sequence similarities
Belongs to the GM-CSF family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
see all -
Database links
- Entrez Gene: 12981 Mouse
- SwissProt: P01587 Mouse
- Unigene: 4922 Mouse
Associated products
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SimpleStep ELISA kits
Images
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Typical Standard CurveRepresentative Standard Curve using ab46078
Protocols
References
This product has been referenced in:
- Wang J et al. Mediating macrophage immunity with wogonin in mice with vascular inflammation. Mol Med Rep 16:8434-8440 (2017). Read more (PubMed: 28983597) »
