Overview

  • Product name

    Mouse GM-CSF ELISA Kit (CSF2)
    See all GM-CSF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5.8%
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.1 pg/ml
  • Range

    12.5 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98 87% - 106%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s GM-CSF (CSF-2) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF protein in mouse cell culture supernatant.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Mouse GM-CSF (CSF-2) is a ~25 kDa pleiotropic cytokine produced by a variety of cells (activated T and B cells, macrophages, mast cells, fibroblasts, endothelial cells) during inflammation or in response to cytokines. GM-CSF can induce proliferation and differentiation of myeloid progenitor cells into eosinophils, macrophages/monocytes, and neutrophils. It is also a survival factor for mature eosinophils, macrophages/monocytes, and neutrophils.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab201276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse GM-CSF (CSF2) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of GM-CSF were measured in duplicate and interpolated from the GM-CSF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 289.7 pg/mL in mouse RAW LPS-stimulated supernatant.

  • RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 µg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. GM-CSF was measured in undiluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7. Measured values were interpolated from the GM-CSF Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed: 4.1 pg/mL unstimulated, 269.5 pg/mL stimulated.

Protocols

References

ab201276 has not yet been referenced specifically in any publications.

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