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  1. Link

    mouse-goat-igg-for-ip-hrp-ab157532.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Mouse Anti-goat IgG for IP (HRP) (ab157532)

  • Datasheet
Submit a review Submit a question References (7)

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Key features and details

  • Mouse Anti-goat IgG for IP (HRP)
  • Conjugation: HRP
  • Host species: Mouse
  • Isotype: IgG
  • Suitable for: WB

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Reagent
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VeriBlot for IP Detection Reagent (HRP) (ab131366)

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Overview

  • Product name

    Mouse Anti-goat IgG for IP (HRP)
    See all IgG secondary antibodies
  • Host species

    Mouse
  • Target species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 0.1% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    eB270
  • Isotype

    IgG
  • General notes

    Number of blots:
    At least 20 blots (based on a 1 µl/ml dilution in 5 ml milk).

    Important protocol notes:

    1. The anti-goat VeriBlot for IP secondary antibody (HRP) detects goat IgG antibodies.

    2. The anti-goat VeriBlot for IP secondary antibody (HRP) preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured forms.

    3. IP sample should be completely reduced/denatured before loaded onto a western blot.

    4. Milk should be used as the blocking protein for the immunoblot.

    Western blot and IP resources:
    a) Western blot a beginner's guide
    b) IP protocol
    c) IP troubleshooting tips

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. The dilution will depend on the sensitivity of the HRP substrate. The recommended dilution is 1 µl in 1 ml milk. Based on the recommended dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples.
Notes
WB
Use at an assay dependent concentration. The dilution will depend on the sensitivity of the HRP substrate. The recommended dilution is 1 µl in 1 ml milk. Based on the recommended dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (7)

Publishing research using ab157532? Please let us know so that we can cite the reference in this datasheet.

ab157532 has been referenced in 7 publications.

  • Wang H  et al. CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14. J Cell Mol Med 25:4596-4607 (2021). PubMed: 33733587
  • Zhang Y  et al. Long non-coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA-224-5p/tumor protein D52 axis. Mol Med Rep 18:4239-4246 (2018). PubMed: 30221720
  • Armelloni S  et al. NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites. Cell Physiol Biochem 46:873-889 (2018). PubMed: 29669318
  • Peng F  et al. PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52:945-954 (2018). PubMed: 29393396
  • Grimaldo L  et al. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. PLoS One 12:e0185289 (2017). PubMed: 28957379
  • Feng G  et al. microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway. Mol Med Rep 14:1631-5 (2016). WB . PubMed: 27314868
  • Chen Q  et al. Chitosan prevents adhesion during rabbit flexor tendon repair via the sirtuin 1 signaling pathway. Mol Med Rep 12:4598-603 (2015). PubMed: 26126698

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