Overview

  • Product name

    Mouse GSDMD ELISA Kit
    See all GSDMD kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Gasdermin D 8 3.6%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2 pg/ml
  • Range

    39.06 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 101.2 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    GSDMD in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Gasdermin D protein in mouse cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Gasdermin D is a key component of pyroptosis. It is activated by inflammasome caspases in response to bacterial infection. Cleaved protein promotes plasma membrane pore formation and subsequent release of pro-inflammatory cytokines into the extra-cellular matrix.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    10X Mouse GSDMD Capture Antibody 1 x 600µl
    10X Mouse GSDMD Detector Antibody 1 x 600µl
    Mouse GSDMD Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Alternative names

    • 1810036L03Rik
    • DF 5L
    • DF5L
    • DFNA 5L
    • DFNA5L
    • FKSG 10
    • FKSG10
    • FLJ12150
    • Gasdermin
    • Gasdermin D
    • Gasdermin domain containing 1
    • Gasdermin domain containing protein 1
    • Gasdermin domain-containing protein 1
    • Gasdermin-D
    • GasderminD
    • GSDMD
    • GSDMD_HUMAN
    • GSDMDC 1
    • GSDMDC1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab233627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentration of Gasdermin D was measured in duplicate, interpolated from the Gasdermin D standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Gasdermin D concentration was determined to be 1684 pg/mL in J774A1.1 cell extracts.

Protocols

References

ab233627 has not yet been referenced specifically in any publications.

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