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|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam's Mouse Haptoglobin ELISA kit an in-vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Haptoglobin in mouse serum, plasma and other biological samples.
In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Haptoglobin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Haptoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Haptoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Haptoglobin in the test sample. The quantity of Haptoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-mouse Haptoglobin antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Mouse Haptoglobin Calibrator (lyophilized)||1 vial|
|Mouse Haptoglobin ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"