Overview

  • Product name
    Mouse heart normal tissue lysate - total protein
    See all Heart lysates
  • Description
    Mouse heart total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    Constituents: 1% Tergitol-NP40, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% SDS, HEPES, Sucrose, 10% Glycerol

    With Protease inhibitor cocktail/ EDTA/Sodium deoxycholate
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab30291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. Unfortunately, I would not recommend using ab66470 as a positive control in Western blot: It is a synthetic peptide derived from within residues 600 - 700 of Human beta-cardiac myosin heavy chain. This peptide was used as an immunogen for an antibody and is only 20 amino acids in length. Therefore, it is hardly suitable for Western blot. I apologise for the misleading information on our datasheet. Indeed, we do have two anti-MYH7 (heavy chain cardiac Myosin) antibodies available in our catalog: ab15 and ab50967 which are guaranteed to react with myosins with the indicated affinities. All tested applications and species are specified on our datasheets, which are updated as soon as any new information is brought to our attention. Please find more detailed information here: Click here (or use the following: https://www.abcam.com/index.html?datasheet=15). Click here (or use the following: https://www.abcam.com/index.html?datasheet=50967). Although we do not have a suitable purified control protein available, I would suggest one of the following lysates. The above mentioned antibodies have been shown to give positive signals with these tissues. Furthermore, these lysates can be of use in future experiments as a positive control for other proteins than myosin as well. ab3869: Click here (or use the following: https://www.abcam.com/index.html?datasheet=3869). ab30291: Click here (or use the following: https://www.abcam.com/index.html?datasheet=30291). I am pleased to let you know that all our products are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. Furthermore, we do not currently have an image of ab15 in Western blot. Therefore, I can offer a discount off a future purchase if you buy ab15 now, use it Western blot and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody. If you are interested in details of this offer, I would be pleased to send you more information on request. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up