Product nameMouse heart tissue lysate - total protein (14 days)
See all Heart lysates
DescriptionMouse heart total protein lysate
General notesSource: Outbred NIH-Swiss Albino mice (14 days old)
The mouse heart tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration information loading...
Our Abpromise guarantee covers the use of ab7194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Ready to load; 10-20 µg/lane is recommended for mini gel.|
ab7194 has not yet been referenced specifically in any publications.