Product nameMouse heart tissue lysate - total protein (7 days)
See all Heart lysates
DescriptionMouse heart total protein lysate
General notesSource: Outbred NIH-Swiss Albino mice (7 days old)
The mouse heart tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.80
Constituent: 5% Beta mercaptoethanol
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Our Abpromise guarantee covers the use of ab7196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. 10 µg to 20 µg per lane is recommended for mini gel.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7196 has not yet been referenced specifically in any publications.