Overview

  • Product name
    Mouse Heme Oxygenase 1 ELISA Kit
    See all HO 1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 2.6%
  • Sample type
    Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    10 pg/ml
  • Range
    78.1 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 105 102% - 111%
    Serum 96 94% - 98%
    Tissue Extracts 110 104% - 113%
    Cell culture media 112 108% - 119%
    Heparin Plasma 88 86% - 91%
    EDTA Plasma 89 87% - 91%
    Citrate Plasma 95 87% - 108%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rabbit, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Heme Oxygenase 1 (HO 1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Heme Oxygenase 1 protein in mouse serum, plasma, urine, cell culture supernatant, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

     

    Sensitivity:

    Samples diluted in Sample Diluent NS: 10 pg/mL

    Samples diluted in 1X Cell Extraction Buffer PTR: 17 pg/mL

  • Notes

    Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 47% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab204524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • ELISA Protocol Summary
  • The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 10,670 pg/mL in mouse serum and 6,084 pg/mL in mouse splenocyte supernatant.

  • The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 5,536 pg/mL in mouse plasma (EDTA), 6,400 pg/mL in mouse plasma (heparin), and 5,479 pg/mL in mouse plasma (Citrate).

  • Interpolated values of Heme Oxygenase 1 are plotted for the indicated cell extracts (y-axis) against the total extract load (x-axis).

Protocols

References

This product has been referenced in:
  • Loboda A  et al. Kidney injury by cyclosporine A is aggravated in heme oxygenase-1 deficient mice and involves regulation of microRNAs. Acta Biochim Pol 65:613-620 (2018). Read more (PubMed: 30481230) »
  • Fan M  et al. DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop. Front Immunol 9:2762 (2018). Read more (PubMed: 30538709) »
See all 5 Publications for this product

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